Biotechnology Classroom Supplies
Sigma Aldrich Fine Chemicals Biosciences CRISPR Non-Target Negative1EA
Recent publications using CRISPR/Cas9-mediated recombineering in E. coli tout editing efficiencies near 100% making CRISPR/Cas9-mediated recombineering the most powerful bacterial genome engineering method to date. In addition Cas9-mediated recombineering overcomes the dependence on a second recombination step avoids the creation of destabilizing scar sites can be used in multiplexing and is less time-consuming than previous protocols.Here we present a novel dual-vector CRISPR/Cas-mediated»-Red system for improved recombineering in E. coli. Our system is shown to facilitate homology-directed repair of DSBs created by Cas9 endonuclease enabling genetic alterations through chromosomal integration of a donor DNA.This plasmid is to be used in combination with the Cas9 Lambda Red homologous recombination plasmid for E. coli (CAS9BAC1P) as the negative control for your custom gene editing experiment. The custom gRNA (CRISPRBACD) can be designed and ordered through https//www.sigmaald
Abcam Pig TNF alpha ELISA Kit, 1 x 96 Tests
Pig TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Pig TNF alpha in Pig in Plasma, Cell culture supernatant, Serum samples.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Pig IL-1 beta ELISA Kit, 1 x 96 Tests
Pig IL-1 beta ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Pig IL-1 beta in Pig in Plasma, Cell culture supernatant, Serum samples.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Pig IL-6 ELISA Kit, 1 x 96 Tests
Pig IL-6 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Pig IL-6 in Pig in Plasma, Cell culture supernatant, Serum samples.
The product is subject to the following: Abcam Restricted Use Statement
Dojindo Molecular Technologies Inc Maleimido-C3-NTA (10MG), CAS: 869843-95-8, can observe the rotation of F1-ATPase with a fluorescence microscope.
Maleimido-C3-NTA is utilized to modify the surfaces on which thiol groups are attached. Through the NTA moiety attached to the surface, genetically expressed protein, bearing a hexahistidine extension at its terminus, can be immobilized via Ni (II) (His-Tag method). Using this technique, Dr. Noji and co-workers were able to directly observe the rotation of F1-ATPase with a fluorescence microscope. Dojindo has various reagents for biosensor research. (https://www.dojindo.com/surface-chemistry/)
Edvotek™ Eucaryotic Cell Biology Experiment Kit
Familiarizes students with simple, robust cell culture system.
3B Scientific™ DNA Double Helix Model
Demonstrates three coils of a DNA double helix with base pairing.
EDVOTEK™ MyLab™ Custom Kit - Building DNA With Beads
Are you planning a virtual curriculum or looking for experiments ideal for distance learning? With the MyLab™ Custom Kits, you can easily plan your curriculum. Each kit comes with instructions and materials for 2-3 runs of each experiment.
EDVOTEK™ MyLab™ Custom Kit - Spooling DNA on a Stick
Are you planning a virtual curriculum or looking for experiments ideal for distance learning? With the MyLab™ Custom Kits, you can easily plan your curriculum. Each kit comes with instructions and materials for 2-3 runs of each experiment.
Edvotek™ Living Art: Adding STEAM to Transformations Kit
Students will harness the color producing power of transformed bacteria to create works of living microbial art. This kit is intended for use in conjunction with Edvotek™ Transformation Kits 222, 223, 224 or 303.